TiBS paper Table 1

Information additional to that published in Trends in Biochemical Sciences, 25(2), 83-88 (2000).

Any researcher having additional information concerning subunit interaction or wishing to make comment
on the following data is asked to contact the author by email.

Table 1 - Subunit-Subunit Interactions of the 19S Regulatory Complex of the 26S Proteasome

Subunit	Other Names	GenBank Number (human)	Domains	Localisation	Interacting Subunits		Methods	Comments	References
					"Rpt/n" nomenclature	"S" nomenclature
Rpt1/ S7		D11094	AAA	B					1, 2
	dm mp42b				Rpt2	S4	P, G	fb, pMss1 res mts2-ts	3, 4
	sc Mss1,Cim5				Rpn1	S2	P	fb	5
					Rpt3	S6b	P	fb, ta	4, 6
					[Rpt1-2-3-6]	[S7 - S4 - S6b - S8]	P	gg	4
						[S7 - S5b* - S4]	P	gg	5
						[S5b* - S7- S4 - S2]	P	gg	5
					Rpn12	S14	G	rpt2-1 sl rpn12-1	7
Rpt2/S4		L02426	AAA	B					8
	h p56				Rpn 1	S2	P, G	pd, mts4-1 sl mts2-1, gg	9, 4
	m M56					[S7 - S4 - S6b - S8]
	sp Mts2				Rpt1	S7
	sc Yhs4					[S4 - S5b* - S7]
Rpt3/S6b		AF038965	AAA	B					10, 11
	h S6, TBP7, h,b p48				Rpt5	S6a	P	ta	10
	ms MS73				Rpt6	S8	P	fb, ta	4, 6
	dm p50				Rpt1	S7
Rpt4/S10b		D78275	AAA	B					12, 13
	h,b p42				Rpt5	S6a	P	fb, gg	4
	sc Sug2,Nas7p				Rpt6	S8	P, G	2hb, sug2-1 sl sug1-1	14
					Rpn2	S1	P	fb	4
						[S6b - S10b - p27*]	P	co-purification	15
Rpt5/S6a		M34079	AAA	B					4, 10
	h TBP1, S6'				Rpn6?	S9?	P?	pd	16
	sc yTbp1				Rpt3, Rpt4	S6b, S10b
Rpt6/S8		D44467	AAA	B					17
	h Trip1, h,b p45				Rpn2	S1	P	gg requires S8 modification	5
	sc Cim3, Sug1				Rpt2, Rpt3, Rpt4	S4, S6b, S10b
Rpn1/S2		D78151	KEKE	B				no KEKE in sp	9, 18
	h TRAP2, h,b p97		9xb/a		Rpn11	S13	G	mts4⁺res pad1-1	19
	sp Mts4				Rpn9	S11	G	mts4-1 sl mts1-1	20
	sc Nas1p				Rpn10	S5a	G	mts4-1 sl pus1D	21
					Rpt1, Rpt2	S7, S4
Rpn2/S1		D44466	KEKE	B					22
	h,b p112		9xb/a		Rpn12	S14	G	sen3D sl nin1-1	22
	sc Sen3p				Rpt4, Rpt6	S10b, S8
Rpn3/S3		D67025	PCI	L					23
	m P91A				Rpn12	S14	G	RPN3 res nin1-1	23
	h p58
	sc Sun2
Rpn5/p55		AB003103	PCI	L					24
	sc Nas5p				none reported
Rpn6/S9		AB003102	PCI	L					25
	b,h p44.5				Rpt5?	S6a?
	sc Nas4
Rpn7/S10a		D14633	KEKE	L					26
	h KIAAORF07		PCI		Rpn8	S12	P	fb	27
Rpn8/S12		D50063	KEKE	L					28
	m Mov34		MPN		Rpn7	S10a
	h,b p40
Rpn9/S11		AB009398	PCI	L					29
	h,b p40.5				Rpn10	S5a	P	2hb	30
	sp Mts1				Rpn12	S14	G	mts1-1 sl mts3-1	20
	sc, Nas7p				Rpn1	S2
Rpn10/S5a		U24704	pUbBD	B/L?					31, 32
	h AF				Rpn11	S13	G	pus1D sl pad1-1	21
	dm p54				Rpn12	S14	G	RPN10 res rpn 12-1	23
	at MBP1						G	pus1D sl mts3-1	21
	sc Sun1				Rpn1, Rpn9	S2, S11
	sp Pus1
Rpn11/S13		U86782	PCI	L					33
	h Poh1				Rpn12	S14	G	pad1-1 sl mts3-1	19
	sp Pad1				Rpn1, Rpn10	S2, S5a
Rpn12/S14		D38047	PCI?	L					34
	h,b p31				Rpt1, Rpn2	S7, S1			24
	sp Mts3				Rpn3, Rpn9	S3, S5a
	sc Nin1				Rpn10, Rpn11	S11, S13
Rpn/t=S. cervisiae nomenclature S=previous (human) nomenclature, h=human, b=bovine, sp=S.pombe, sc=S.cerevsiae, m=mouse, dm=Drosophila melanogaster, at=Arabidopsis thaliana, sm=Manduca sexta, Localisation: B - base subunit, L - lid subunit, Domains: KEKE, AAA, PCI, MPN - see text, 9xb/a=sequence predicted to form a half horseshoe somewhat flat hydrophobic surface, also found in APC complexes and similar to the LRR region in ribonuclease inhibitor (35), pUbBD=polyUbiquitin binding domain, PCI? - assigned a PCI domain because of its weak homology to COP9 signalosome subunit 8 within amino acids 180-210 of S14, [ ]=subcomplexes formed, P=protein-protein interaction, G=genetic interactions, fb=filter binding assays, sl=synthetic lethal, res=multi-copy expression of wild type gene rescues mutation, sup=suppressor mutation analysis, pd=pull down including; glutathion sepharose-gst pull down, Ni-agarose-His pull down, immunoprecipitation, gg=co-sedimentation on glycerol gradients, ts=temperature sensitive, ta=transcriptional activation, 2hb=2hybrid. *=species or tissue specific protein, interaction redundancies.

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